| — | The Universe & I | Sherlock Holmes |
| 2021 | Emily @ the Edge of Chaos | Sigmund Freud (voice) |
| 2008 | The Real Story | Himself |
| — | Symphony to the Planets: A Star to Call Home | Narrator |
| — | Symphony to the Planets: Mysteries Beyond Our Reach | Narrator |
| 2018 | My Brother Vincent | Self |
| 2013 | Leonard Nimoy's Oral History | Self - Interviewee |
| — | Leonard Nimoy Demonstrates the Magnavision Videodisc Player | Self |
| 2023 | Time Bomb Y2K | Self (archive voice) |
| 2021 | Star Trek: Prodigy | Spock (voice) |
| 2021 | The Center Seat: 55 Years of Star Trek | Self (archive footage) |
| 2017 | Star Trek: Discovery | Spock (archive footage) |
| 2016 | The Shannara Chronicles | Spock (archive footage) |
| 2016 | For the Love of Spock | Self (archive footage) |
| 2016 | 50 Years of Star Trek | Self |
| 2016 | Star Trek: Inside the Roddenberry Vault | (archive footage) |
| 2014 | To Be Takei | Self |
| 2013 | Into Darkness - Star Trek | Ambassador Spock |
| 2013 | The Prime Ministers: The Pioneers | Levi Eshkol (voice) |
| 2012 | Zambezia | Sekhuru (voice) |
| 2012 | Hava Nagila: The Movie | Self |
| 2011 | Transformers 3 | Sentinel Prime (voice) |
| 2011 | Billy Blackburn's Treasure Chest: Rare Home Movies and Special Memories | |
| 2011 | Classic TV Bloopers Uncensored | Himself |
| 2009 | Star Trek - Il futuro ha inizio | Ambassador Spock |
| 2009 | Land of the Lost | The Zarn (voice) |
| 2009 | Late Night with Jimmy Fallon | Self |
| 2009 | Star Trek: The Captains' Summit | Self |
| 2009 | Star Trek: Evolutions | Narrator |
| 2009 | The Evolution of the Enterprise | Self |
| 2009 | Bring Back... Star Trek | Self |
| 2008 | Fringe | Dr. William Bell |
| 2008 | Shatner's Raw Nerve | Self |
| 2007 | The Big Bang Theory | Mr. Spock (voice) |
| 2007 | The Pixar Story | Spock (archive footage) |
| 2007 | Star Trek: Beyond the Final Frontier | Self - Host |
| 2006 | 40 Years of Star Trek | Self |
| 2006 | William Shatner in Concert | Self |
| 2006 | The Once and Future Griffith Observatory | Self |
| 2005 | Ringers: Lord of the Fans | Self |
| 2005 | Bring Back... | Self |
| 2005 | Rambam - The Story of Maimonides | Rambam (voice) |
| 2004 | Reflections on Spock | |
| 2004 | Kiss 'N' Tell: Romance in the 23rd Century | |
| 2004 | Birth of a Timeless Legacy | |
| 2003 | Comedy Central Roasts | Self |
| 2002 | The Making of 'Atlantis: The Lost Empire' | Self |
| 2002 | HypaSpace | Self |
| 2002 | After They Were Famous | Self |
| 2001 | Atlantis - L'impero perduto | King Kashekim Nedakh (voice) |
| 2001 | Mind Meld: Secrets Behind the Voyage of a Lifetime | Himself |
| 2000 | Sinbad - Un'avventura di spada e magia | Akron/ Baraba/ King Chandra |
| 2000 | Forging Greatness: Lincoln in Indiana | Narrator |
| 2000 | The Fly Papers: The Buzz on Hollywood's Scariest Insect | Narrator |
| 1999 | Futurama | Leonard Nimoy's Head (Voice) |
| 1999 | Y2K Family Survival Guide | Self - Host / Narrator |
| 1999 | The Story of Computer Graphics | Narrator |
| 1999 | Rashi: A Light After The Dark Ages | Rashi (voice) |
| 1998 | Becker | Professor Emmett Fowler |
| 1998 | Brave New World | Mustapha Mond |
| 1998 | Invasion America | |
| 1998 | A Life Apart: Hasidism in America | Narrator |
| 1998 | The Lost World | Angus McArdle |
| 1997 | Trekkies | Self |
| 1997 | Davide | Samuel |
| 1997 | The Harryhausen Chronicles | Self - Narrator (voice) |
| 1997 | The Great Pyramids | Narrator (voice) |
| 1996 | The Daily Show | Self |
| 1996 | Muppets Tonight | Self |
| 1996 | Star Trek: 30 Years and Beyond | Self |
| 1996 | Star Trek Story | Self |
| 1996 | Jellies & Other Ocean Drifters | Narrator |
| 1996 | Mystical World of Ancient Greece | Narrator (voice) |
| 1995 | Oltre i limiti | Thurman Cutler |
| 1995 | Star Trek: The Next Generation: Il segreto di Spock (Parte I e II) | Spock (archive footage) |
| 1995 | Bonanza: Under Attack | Frank James |
| 1995 | William Shatner's Star Trek Memories | Self |
| 1995 | The New Explorers: The Science of Star Trek | Self |
| 1995 | Mysterious Spirits of the Mayans | Narrator (voice) |
| 1994 | Pagemaster - L'avventura meravigliosa | Dr. Jekyll / Mr. Hyde (voice) |
| 1994 | Duckman | |
| 1994 | Ancient Mysteries | |
| 1994 | Ancient Mysteries | Narrator |
| 1994 | Destiny in Space | Narrator (voice) |
| 1994 | Star Trek: A Captain's Log | Himself |
| 1994 | The Making of 'The Pagemaster' | Self (archive footage) |
| 1994 | Science Fiction: A Journey Into the Unknown | |
| 1994 | William Shatner: Captain's Log | Self |
| 1994 | Ancient Mysteries: Bigfoot | Host |
| 1993 | The Halloween Tree | Mr. Moundsroud (Voice) |
| 1992 | MTV Movie & TV Awards | Spock (archive footage) |
| 1992 | Titanica | narrator |
| 1991 | Star Trek VI - Rotta verso l'ignoto | Captain Spock |
| 1991 | Star Trek: 25th Anniversary Special | Self - Host |
| 1991 | Never Forget | Mel Mermelstein |
| 1991 | Haunted Lives: True Ghost Stories | Host |
| 1989 | I Simpson | Leonard Nimoy (voice) |
| 1989 | Star Trek V - L'ultima frontiera | Captain Spock |
| 1988 | The Star Trek Saga: From One Generation to the Next | Self |
| 1987 | Star Trek: The Next Generation | Spock |
| 1986 | Star Trek IV - Rotta verso la Terra | Captain Spock |
| 1986 | The Transformers: The Movie | Galvatron (voice) |
| 1986 | Aladdin and His Wonderful Lamp | The Evil Moroccan Magician |
| 1986 | Super Duper Bloopers | Himself |
| 1985 | Hollywood's Funniest All-Star Bloopers | Self (archive footage) |
| 1985 | Planets of the Sun | Narrator |
| 1984 | Star Trek III - Alla ricerca di Spock | Captain Spock / Elevator Voice |
| 1984 | The Sun Also Rises | Count Mippipopolous |
| 1984 | Lights: The Miracle of Chanukah | Greek Nobleman |
| 1983 | Leonard Nimoy: Star Trek Memories | Host |
| 1982 | Star Trek II - L'ira di Khan | Captain Spock |
| 1982 | T. J. Hooker | Lt. Paul McGuire |
| 1982 | Marco Polo | Achmet |
| 1982 | Il castello delle fiabe | The Evil Moroccan Magician |
| 1982 | A Woman Called Golda | Morris Meyerson |
| 1982 | Wogan | Self |
| 1982 | Night of 100 Stars | Self |
| 1981 | Vincent | Theo van Gogh |
| 1980 | Seizure: The Story of Kathy Morris | Dr. Richard Connought |
| 1979 | Star Trek | Mr. Spock |
| 1978 | Terrore dallo spazio profondo | Dr. David Kibner |
| 1977 | In Search of... | |
| 1977 | In Search of... | Narrator |
| 1977 | Bitte umblättern | Self |
| 1977 | Auf los geht's los | Self |
| 1976 | The Captain and Tennille | Self |
| 1975 | Saturday Night Live | Self - Cameo (uncredited) |
| 1975 | The Star Trek Dream | Self |
| 1975 | The Missing Are Deadly | |
| 1975 | Mitzi & 100 Guys | Self |
| 1974 | Rex Harrison Presents Stories of Love | Mick |
| 1973 | Star Trek - La serie animata | Spock (voice) |
| 1973 | Una testa di lupo mozzata | Tom Kovack |
| 1973 | L'ultimo colpo dell'ispettore Clark | Mitch |
| 1971 | Colombo | Barry Mayfield |
| 1971 | Catlow | Miller |
| 1971 | Assault on the Wayne | Comdr. Phil Kettenring |
| 1970 | Mistero in galleria | Henry Auden |
| 1969 | The Barbara McNair Show | Self |
| 1968 | The Beautiful Phyllis Diller Show | Self |
| 1967 | The Carol Burnett Show | Self - Guest / Various Characters |
| 1967 | Rowan & Martin's Laugh-In | Self |
| 1967 | Valley of Mystery | Spence Atherton |
| 1967 | Malibu U | Self |
| 1966 | Star Trek | Spock |
| 1966 | Missione Impossibile | Paris |
| 1966 | Deathwatch | Jules LaFranc |
| 1965 | Get Smart | |
| 1965 | Star Trek: The Cage | Spock |
| 1964 | Organizzazione U.N.C.L.E. | |
| 1964 | Daniel Boone | Oontah |
| 1964 | The Hollywood Palace | Self - Singer |
| 1964 | Profiles in Courage | Burr Jones |
| 1963 | Oltre i limiti | Konig |
| 1963 | Oltre i limiti | Judson Ellis |
| 1963 | The Balcony | Roger |
| 1963 | Kraft Suspense Theatre | Lawrence Brody |
| 1963 | Kraft Suspense Theatre | Cowell |
| 1963 | The Lieutenant | Gregg Sanders |
| 1963 | You Don't Say! | |
| 1962 | Combat! | Neumann |
| 1962 | Combat! | Pvt. Baum |
| 1962 | Il virginiano | Wismer |
| 1962 | Il virginiano | Benjamin Frome |
| 1962 | Il virginiano | Keith Bentley |
| 1962 | Sam Benedict | |
| 1961 | Dottor Kildare | Harry |
| 1961 | The Mike Douglas Show | Self |
| 1961 | 87° squadra | |
| 1961 | Lotta senza quartiere | Ralph Tomek |
| 1960 | The Tall Man | |
| 1960 | The Tall Man | Deputy Sheriff Johnny Swift |
| 1960 | Tate | |
| 1959 | Ai confini della realtà | Hansen |
| 1959 | Bonanza | Freddy |
| 1959 | Gli uomini della prateria | Anko |
| 1959 | The Rebel | Jim Colburn |
| 1959 | Lock-Up | |
| 1958 | The Brain Eaters | Professor Cole |
| 1958 | L'uomo del mare | |
| 1958 | The Rough Riders | |
| 1958 | Satan's Satellites | Narab |
| 1957 | Perry Mason | Pete Chennery |
| 1957 | Carovane verso il west | Bernabe Zamora |
| 1957 | 26 Men | |
| 1957 | Harbor Command | |
| 1956 | Broken Arrow | Winnoa |
| 1956 | West Point | |
| 1956 | West Point | Cadet Tom Kennedy |
| 1956 | Man Called X | |
| 1955 | Gunsmoke | Grice |
| 1955 | Gunsmoke | Arnie |
| 1955 | Gunsmoke | Holt |
| 1955 | Gunsmoke | John Walking Fox |
| 1955 | La pattuglia della strada | |
| 1955 | Navy Log | |
| 1954 | Assalto alla Terra | Army Sergeant |
| 1953 | Old Overland Trail | Chief Black Hawk |
| 1952 | Francis all'accademia | Football Player |
| 1952 | Zombies of the Stratosphere | Narab |
| 1952 | Kid Monk Baroni | Paul 'Monk' Baroni |
| 1951 | Dragnet | |
| 1951 | Il gatto milionario | Young Baseball Player (uncredited) |
| 1951 | Sogni ad occhi aperti | Chief |
