| — | Machete Kills Again... in Space | Machete |
| — | Downtown Bus | Sancho 'The Jailbird' Sanchez |
| — | From a Son | George |
| — | Pug | |
| — | The Damned | Chimalle |
| — | Occupation Rainfall: Chapter 2 | |
| 2024 | The Night They Came Home | Digger |
| 2024 | Mr. Birchum | Switchblade (voice) |
| 2024 | Detour 95 | Muerte |
| 2024 | The Legend of Johnny Jones | El Muerte |
| 2024 | The Street Avenger | Muerte |
| 2024 | Tim Travers & the Time Travelers Paradox | Royce |
| 2024 | NHL Big City Greens Classic 2 | Vasquez (voice) |
| 2023 | The Muppets Mayhem | Self |
| 2023 | Miami Bici 2 | Mr Susan |
| 2023 | Death on the Border | Father Francis |
| 2022 | Minions: The Rise of Gru | Stronghold (voice) |
| 2022 | Clerks III | Auditioner |
| 2022 | La Llorona | Jorge |
| 2022 | It's a Wonderful Binge | Angel |
| 2022 | Good Mourning | Method Cameo |
| 2022 | Renegades | Sanchez |
| 2022 | A Tale of Two Guns | Junior |
| 2022 | Wolf Mountain | Eddie |
| 2022 | Lopez vs Lopez | |
| 2022 | Iron Chef: Quest for an Iron Legend | Self - Guest Judge |
| 2022 | Goodbye Brooklyn Nine-Nine | Oscar Diaz (archive footage) |
| 2022 | The Prey | Vega |
| 2022 | Green Ghost and the Masters of the Stone | Master Gin |
| 2022 | PBC | Ricky |
| 2021 | The Book of Boba Fett | Rancor Keeper |
| 2021 | Monsters at Work | Terry (voice) |
| 2021 | American Horror Stories | Santa |
| 2021 | Masters of the Universe: Revelation | Ram Man (voice) |
| 2021 | Maya and the Three | Cabrakan (voice) |
| 2021 | Muppets Haunted Mansion | Ghost of Huet |
| 2021 | American Sicario | Pedro |
| 2021 | Pups Alone | Vinnie P (voice) |
| 2021 | The House Next Door: Meet the Blacks 2 | Self |
| 2021 | La sombra del gato | Sombra |
| 2021 | The Ghost and Molly McGee | Bobby Daniels (voice) |
| 2021 | The Rebels of PT-218 | Seaman Edgar 'Cookie' Orozco |
| 2021 | Total Badass Wrestling | Bobby 'The Body' Estrada |
| 2020 | The SpongeBob Movie: Sponge on the Run | El Diablo |
| 2020 | American Pie Presents: Girls' Rules | Janitor Steve Garcia |
| 2020 | Bullets of Justice | Gravedigger |
| 2020 | Pistolera | Indio |
| 2020 | LA Originals | Self |
| 2020 | Muppets Now | Self |
| 2020 | Final Kill | Francesco |
| 2020 | The Last Exorcist | Marco |
| 2020 | Lumpia: With a Vengeance | Reyes |
| 2020 | Paragon: The Shadow Wars | Kincaid |
| — | American Underdog | |
| — | Seven Cemeteries | Bravo |
| — | The Wrecker | |
| 2024 | I Heart Robots | Bob |
| 2024 | Gracie and Pedro: Pets to the Rescue | Laurence (voice) |
| 2023 | Hunting Games | Sheriff Ramos |
| 2023 | Una preghiera per Giuda | Carnizzari |
| 2023 | 1521: The Quest for Love and Freedom | Ferdinand Magellan |
| 2023 | The Dream Americano | Self |
| 2022 | VampFather | Vampfather |
| 2022 | Caroltyn | Wells |
| 2022 | Smile or Hug | |
| 2022 | Escándalo Secreto En Plena Cuarentena | MMR, Mario Machin Rin |
| 2022 | Fire-Man's Fetish | Fire-Man |
| 2021 | Donny's Bar Mitzvah | Self |
| 2021 | Death Rider in the House of Vampires | Bela Latigo |
| 2021 | The Black Hole | Edmund |
| 2021 | Comedy Chingonas | Self - Host |
| 2021 | Quentin Tarantino: From a Movie Buff to a Hollywood Legend | Video footage |
| 2020 | Cover Me | Big Mike |
| 2020 | HA Festival: The Art of Comedy | |
| 2019 | Fast & Furious Spy Racers | Tuco (voice) |
| 2019 | Dora and the Lost City of Gold | Boots (voice) |
| 2019 | What We Do in the Shadows | Danny |
| 2019 | 3 from Hell | Rondo |
| 2019 | L.A.'s Finest | Reuben Velazquez |
| 2019 | The Masked Singer | Raccoon |
| 2019 | The Kelly Clarkson Show | Self |
| 2019 | Acceleration | Santos |
| 2019 | Seis Manos | Baldemar / El Balde (voice) |
| 2019 | The Short History of the Long Road | Miguel |
| 2019 | Madness in the Method | Danny |
| 2019 | Grand-Daddy Day Care | Eduardo Hernandez |
| 2019 | Wish Man | Jose |
| 2019 | Inmate #1: The Rise of Danny Trejo | Self |
| 2019 | Big Kill | General Morales |
| 2019 | The Outsider | Carlos |
| 2019 | Victor and Valentino | Paco (voice) |
| 2019 | Victor and Valentino | The Cupcake Man (voice) |
| 2019 | Slayer: The Repentless Killogy | Danny |
| 2019 | Lights Out with David Spade | Self |
| 2019 | Slasher Party | Danny Trejo |
| 2019 | Cross: Rise of the Villains | Muerte |
| 2019 | Counterpunch | Manny Navarro |
| 2019 | Bare Knuckle Brawler | Santo Ariza |
| 2019 | To Hell and Back: The Making of 3 From Hell | Himself |
| 2019 | Theme Song Takeover | Vasquez (voice) |
| 2019 | The Margarita Man | Dr. Valenzuela |
| 2019 | Every 9 Hours | Raul |
| 2019 | Black Licorice | Donicio |
| 2018 | Death Race: Beyond Anarchy | Goldberg |
| 2018 | The Conners | Tito |
| 2018 | Big City Greens | Vasquez (voice) |
| 2018 | Survivor's Guide to Prison | Self |
| 2018 | Gone Are the Days | River Man |
| 2018 | American Nightmares | Mister Malevolent |
| 2018 | Frat Pack | Dirty |
| 2018 | Silencer | Lenard "Big Daddy" Ochoa |
| 2018 | Bully | Manny |
| 2018 | #Roxy | Principal Castillo |
| 2018 | Fury of the Fist and the Golden Fleece | Nino Grande |
| 2018 | Illegal Civilization 3 | Self |
| 2017 | American Gods | Mr. World |
| 2017 | Puss in Book: Trapped in an Epic Tale | El Moco (voice) |
| 2017 | Dead Again in Tombstone | Guerrero |
| 2017 | All About the Money | Luis Diego |
| 2017 | Cross Wars | Murete |
| 2017 | Максимальный удар | Don Sanchez |
| 2017 | Murder in the Woods | Sheriff Lorenzo |
| 2017 | Juarez 2045 | Angel Malvado |
| 2017 | Avenge the Crows | Antonio |
| 2017 | McDick | Oscar |
| 2017 | Not a War Story | |
| 2017 | BorderCross | |
| 2017 | Calico Queens | Bill Gallows |
| 2016 | Storks | Jasper (voice) |
| 2016 | Angie Tribeca | Himself |
| 2016 | Cyborg X | Captain Machine Gun |
| 2016 | Mostly Ghostly 3: One Night in Doom House | Mr. Morgo |
| 2016 | Range 15 | Zombie Trejo |
| 2016 | Vigilante Diaries | Crazy Joe |
| 2016 | Halloweed | Patch |
| 2016 | Boost | Roy Casares |
| 2016 | 1st Strike | Manny Garcia |
| 2016 | North by El Norte | Uncle John |
| 2016 | Map of Hell | Himself |
| 2016 | Street Level | Carlton the Doorman |
| 2016 | Bridesman | Eduardo |
| 2016 | Chronology | Jackhammer |
| 2016 | Good Fortune | Self |
| 2015 | The Ridiculous 6 | Cicero |
| 2015 | The Late Show with Stephen Colbert | |
| 2015 | 3-Headed Shark Attack | Max Burns |
| 2015 | New Looney Tunes | Lieutenant (voice) |
| 2015 | Bad Asses on the Bayou | Frank Vega |
| 2015 | 4Got10 | Mateo Perez |
| 2015 | The Adventures of Puss in Boots | El Moco (voice) |
| 2015 | The Night Crew | Aguilar |
| 2015 | Real Rob | Danny |
| 2015 | VANish | Carlos |
| 2015 | Hope Lost | Marius |
| 2015 | L.A. Slasher | The Drug Dealer #2 |
| 2015 | The Burning Dead | Night Wolf |
| 2015 | Smoke and Mirrors: The Story of Tom Savini | Self |
| 2015 | A Horse Story | Moonlight (voice) |
| 2015 | Sock 'Em Dead | Self - Host |
| 2015 | Pig Goat Banana Cricket | Filthy the Foot (voice) |
| 2015 | No Way Out | Don Caceres |
| 2015 | Alcatraz Prison Escape: Deathbed Confession | Narrator (voice) |
| 2015 | Fluffy's Food Adventures | Himself |
| 2015 | Teenage Mutant Ninja Turtles: Renegade Rampage | Newtralizer (voice) |
| 2015 | The Weight of Blood and Bones | Espada |
| 2015 | East LA Interchange | narrator |
| 2014 | The Flash | Breacher |
| 2014 | The Book of Life | Skeleton Luis (voice) |
| 2014 | Muppets Most Wanted | Danny Trejo |
| 2014 | In the Blood | Big Biz |
| 2014 | From Dusk Till Dawn: The Series | The Regulator |
| 2014 | Bad Ass 2: Bad Asses | Frank Vega |
| 2014 | Bullet | Frank |
| 2014 | Reach Me | Vic |
| 2014 | Force of Execution | Oso |
| 2014 | Gun | Frankie Makina |
| 2014 | Scooby-Doo! Ghastly Goals | Reynaldo (voice) |
| 2014 | Reaper | Jack |
| 2014 | Running Wild with Bear Grylls | Self |
| 2014 | Preggoland | Pedro |
| 2014 | Voodoo Possession | Kross |
| 2014 | 20 Ft Below: The Darkness Descending | Angel |
| 2014 | Beyond Justice | Tattoo |
| 2014 | Strike One | Manny Garcia |
| 2014 | Misfit Garage | |
| 2014 | Saint George | Tio |
| 2014 | Beyond the Game | José |
| 2014 | Habla Men | Self |
| 2014 | Vieques | |
| 2013 | Rick and Morty | Jaguar (voice) |
| 2013 | Brooklyn Nine-Nine | Oscar Diaz |
| 2013 | Machete Kills | Machete Cortez |
| 2013 | Death Race: Inferno | Goldberg |
| 2013 | Mickey Mouse | Piñata Boss (voice) |
| 2013 | Dead in Tombstone | Guerrero |
| 2013 | Phineas and Ferb: Mission Marvel | Venom (voice) |
| 2013 | Zombie Hunter | Jesús |
| 2013 | Anthony Bourdain: Parts Unknown | Self |
| 2013 | The Contractor | Javier Reyes |
| 2013 | Билет на Vegas | Mister Chich |
| 2013 | Five Thirteen | El Loco |
| 2013 | The Insomniac | Jairo Torres |
| 2013 | Circus Halligalli | |
| 2013 | Tattoo Nation | Himself |
| 2013 | Курьер из 'Рая' | |
| 2013 | Chavez Cage of Glory | Mando |
| 2013 | American Lowrider | The O.G. |
| 2013 | Pendejo | Pedro |
| 2013 | Amelia's 25th | Don Javier |
| 2012 | Bad Ass | Frank Vega |
| 2012 | Breaking Wind | Billy Black |
| 2012 | Recoil | Drayke |
| 2012 | Sushi Girl | Schlomo |
| 2012 | Rise of the Zombies | Caspian |
| 2012 | Haunted High | Ortiz |
| 2012 | The Cloth | Father Connely |
| 2012 | Bro' | Gilbert |
| 2012 | Mango Bajito | Carson |
| 2011 | Spy Kids: All the Time in the World | Uncle Machete |
| 2011 | Haroldi ja Kumari jõulud | Mr. Perez |
| 2011 | Violet & Daisy | Russ |
| 2011 | House of the Rising Sun | Carlos |
| 2011 | Cross | Lexavier |
| 2011 | Poolboy: Drowning Out the Fury | Ceasar |
| 2011 | The Black Mamba | The Crippler |
| 2011 | Blacktino | Rogelio |
| 2011 | In the Shadow | Hermit |
| 2011 | Dark Games | Archie |
| 2010 | Predators | Cuchillo |
| 2010 | Machete | Machete |
| 2010 | Young Justice | Bane (voice) |
| 2010 | Death Race 2 | Goldberg |
| 2010 | The Killing Jar | Jimmy |
| 2010 | The Good Guys | Alfredo |
| 2010 | Saint John of Las Vegas | Bismarck |
| 2010 | Epic Rap Battles of History | himself |
| 2010 | Shoot the Hero | Crazy Joe |
| 2010 | Beatdown | Marcus |
| 2010 | The Lazarus Papers | Aroon |
| 2010 | Shadows in Paradise | Matador |
| 2010 | Pastor Shepherd | Phil Harrison |
| 2010 | The Bill Collector | Frankie Gutierrez |
| 2010 | Predators: The Chosen | Cuchillo |
| 2010 | Modus Operandi | Director Holiday |
| 2010 | Predators: Moments of Extraction | Cuchillo (Voice) |
| 2010 | Boston Girls | Uncle Reggie |
| 2010 | American Flyer | Uncle John |
| 2010 | Justin Time | Mardok |
| 2009 | Modern Family | Gus |
| 2009 | NCIS: Los Angeles | Tuhon |
| 2009 | Fanboys | The Chief |
| 2009 | RuPaul's Drag Race | Self - Special Guest / Big Daddy |
| 2009 | The Haunted World of El Superbeasto | Rico (voice) |
| 2009 | La Linea | Mario |
| 2009 | Eyeborgs | G-Man |
| 2009 | The Grind | Nicholi Guzman |
| 2009 | The Boys of Ghost Town | Father |
| 2009 | Official Rejection | Self |
| 2009 | Babysitters Beware | Clyde / The Guard |
| 2009 | The Face Is Familiar | Himself |
| 2009 | Cowboy Dreams | Lobo |
| 2008 | Halvale teele | Tortuga |
| 2008 | Sons of Anarchy | Romero 'Romeo' Parada |
| 2008 | The Spectacular Spider-Man | Ox (voice) |
| 2008 | Alone in the Dark 2 | Perry |
| 2008 | The Art of Travel | Limo Driver |
| 2008 | Toxic | Antoine |
| 2008 | Necessary Evil | Barro |
| 2008 | Michael Lives: The Making of Halloween | Self |
| 2008 | The Wendy Williams Show | Self |
| 2008 | Jake's Corner | Clint |
| 2008 | Valley of Angels | Hector |
| 2008 | Ranchero | Capone |
| 2008 | El Superstar: The Unlikely Rise of Juan Frances | E.J. |
| 2008 | D.O.P.E. - Death Or Prison Eventually | Narrator |
| 2008 | Railroad to Hell: A Chinaman's Chance | Manolo |
| 2007 | Planet Terror | Machete |
| 2007 | Halloween | Ismael Cruz |
| 2007 | Grindhouse | Machete (segment "Planet Terror") |
| 2007 | Phineas and Ferb | Venom (voice) |
| 2007 | Burn Notice | Felipe Vega |
| 2007 | Smiley Face | Albert |
| 2007 | Battle for Terra | Elder Barum (voice) |
| 2007 | Delta Farce | Carlos Santana |
| 2007 | Urban Justice | El Chivo |
| 2007 | Tim and Eric Awesome Show, Great Job! | Criminal |
| 2007 | Blood Ties | Pacha Kamaq |
| 2007 | El Tigre: The Adventures of Manny Rivera | |
| 2007 | Heckler | Self |
| 2007 | Furnace | Fury |
| 2007 | Machete | Machete |
| 2007 | On Bloody Sunday | The Ref |
| 2007 | The Blue Rose | Junk |
| 2007 | Richard III | Major |
| 2006 | Sherrybaby | Dean Walker |
| 2006 | Snoop Dogg's Hood of Horror | Derelict |
| 2006 | Top Chef | Himself - Guest Judge |
| 2006 | 7 Mummies | Apache |
| 2006 | Slayer | Montegna |
| 2006 | National Lampoon's TV: The Movie | Crow |
| 2006 | Death Row | Priest |
| 2006 | High Hopes | Shady |
| 2006 | Heist | |
| 2006 | Jack's Law | Jack Santos |
| 2006 | Living the Dream | Chuck |
| 2006 | Propensity | Roy |
| 2006 | Tennis Anyone? | Hector |
| 2005 | Bones | Bishop Edward Galano |
| 2005 | Ameerika paps | (voice) |
| 2005 | Ameerika paps | Mexican Thugs (voice) |
| 2005 | The Devil's Rejects | Rondo |
| 2005 | Hell's Kitchen | Self |
| 2005 | The Crow: Wicked Prayer | Harold |
| 2005 | All Souls Day: Dia de los Muertos | Vargas Diaz |
| 2005 | Chasing Ghosts | Carlos Santiago |
| 2005 | The Curse of El Charro | El Charro (Voice) |
| 2005 | Champion | Self |
| 2005 | Venice Underground | Papi |
| 2005 | The Making of 'Heat' | Self |
| 2005 | Unscripted | Self |
| 2005 | 30 Days in Hell: The Making of 'The Devil's Rejects' | Self |
| 2005 | Nobody Wants Your Film | Self |
| 2004 | Anchorman: The Legend of Ron Burgundy | Bartender |
| 2004 | Desperate Housewives | Hector Ramos |
| 2004 | Stargate Atlantis | Omal |
| 2004 | Wake Up, Ron Burgundy: The Lost Movie | Bartender |
| 2004 | Lost | Edward James Archer |
| 2004 | Turned Out: Sexual Assault Behind Bars | Narrator |
| 2003 | Spy Kids 3-D: Game Over | Machete |
| 2003 | Once Upon a Time in Mexico | Cucuy |
| 2003 | The Big Empty | Lt. Gates |
| 2003 | Kingpin | |
| 2002 | xXx | El Jefe |
| 2002 | Spy Kids 2: The Island of Lost Dreams | Machete |
| 2002 | Monk | Spyder Rudner |
| 2002 | The Salton Sea | Little Bill |
| 2002 | George Lopez | |
| 2002 | Beat the Devil | Bob |
| 2002 | 13 Moons | Hoodlum |
| 2002 | Nightstalker | Officer Frank Luis |
| 2002 | Do It for Uncle Manny | Pedro |
| 2001 | Spy Kids | Machete |
| 2001 | Alias | Emilio Vargas |
| 2001 | Bubble Boy | Slim |
| 2001 | Grounded for Life | |
| 2001 | The Hire | Bob |
| 2001 | Skippy | Hitman |
| 2000 | Reindeer Games | Jumpy |
| 2000 | Animal Factory | Vito |
| 2000 | Aqua Teen Hunger Force | Dewey (voice) |
| 2000 | The District | Self |
| 2000 | Cuenta Saldada | Secuestrador 1 (as Daniel Trejo) |
| 1999 | Perepea | Clint Beltran (voice) |
| 1999 | Perepea | (voice) |
| 1999 | Futurama | Doblando Rodriguez (voice) |
| 1999 | From Dusk Till Dawn 2: Texas Blood Money | Razor Eddie |
| 1999 | From Dusk Till Dawn 3: The Hangman's Daughter | Razor Charlie |
| 1999 | Inferno | Johnny Six Toes |
| 1999 | Whiteboyz | Prisoner |
| 1999 | Soundman | Duce's Father |
| 1998 | Kuus päeva, seitse ööd | Pierce |
| 1998 | The Replacement Killers | Collins |
| 1998 | The Crow: Stairway to Heaven | Harold |
| 1998 | Point Blank | Wallace |
| 1998 | Champions | Max Brito |
| 1997 | Con Air | "Johnny-23" |
| 1997 | Anaconda | Poacher |
| 1997 | King of the Hill | Victor Velasquez (voice) |
| 1997 | King of the Hill | Enrique (voice) |
| 1997 | King of the Hill | Lifeguard / Waiter (voice) |
| 1997 | King of the Hill | Enrique / Octavio (voice) |
| 1997 | King of the Hill | (voice) |
| 1997 | King of the Hill | Octavio (voice) (uncredited) |
| 1997 | Trojan War | Scarface |
| 1997 | Brooklyn South | Louis - Hertz's Bodyguard |
| 1997 | Dilemma | Rudy Salazar |
| 1997 | Los Locos | Manuel Batista |
| 1996 | From Dusk Till Dawn | Razor Charlie |
| 1996 | Le Jaguar | Kumare |
| 1995 | Pinge | Trejo |
| 1995 | Desperado | Navajas |
| 1995 | The Stranger | Hawk |
| 1995 | Dead Badge | El Tango Bartender |
| 1994 | Against the Wall | Luis |
| 1994 | Mi Vida Loca | Frank |
| 1994 | High Tide | Tattooist |
| 1994 | Criminal Passion | Construction Worker |
| 1994 | Vanishing Son | Out-of-Breath Man |
| 1994 | Victor One | |
| 1993 | The X-Files | Cesar Ocampo |
| 1993 | Bound by Honor | Geronimo |
| 1993 | Walker, Texas Ranger | Joe Lopez |
| 1993 | Walker, Texas Ranger | José Rodriguez |
| 1993 | NYPD Blue | Gabriel mota |
| 1993 | NYPD Blue | Frankie soto |
| 1993 | 12:01 | Prisoner |
| 1993 | Doppelganger | Hard Hat |
| 1993 | Love, Cheat & Steal | Cuban |
| 1993 | Last Light | 2nd Inmate |
| 1993 | Fallen Angels | Boxer |
| 1993 | Sunset Grill | Young Mexican |
| 1992 | Renegade | Freddie |
| 1992 | Nails | Las Virgenes bartender |
| 1992 | Sex Crimes | Palmer |
| 1991 | Wedlock | Tough Prisoner #1 |
| 1991 | Whore | Tattoo Artist |
| 1991 | Carnal Crimes | Ticket Agent |
| 1991 | Femme Fatale | Toshi |
| 1991 | Reasonable Doubts | Cholo #2 |
| 1991 | Lonely Hearts | Angry Client |
| 1991 | Doublecrossed | Lito |
| 1991 | The Last Hour | Spider |
| 1991 | FBI: The Untold Stories | Snowman |
| 1990 | Marked for Death | Hector |
| 1990 | Maniac Cop 2 | Prisoner |
| 1990 | Guns | Tong |
| 1990 | Drug Wars: The Camarena Story | Gabriel |
| 1989 | Lock Up | Chink's Gang Member |
| 1989 | Baywatch | |
| 1989 | Baywatch | Carlos Urueta |
| 1989 | Kinjite: Forbidden Subjects | Prison Inmate |
| 1989 | Bail Out | Mean |
| 1989 | Cage | Costello's Bodyguard |
| 1988 | Bulletproof | Sharkey |
| 1987 | The Hidden | Prisoner |
| 1987 | Death Wish 4: The Crackdown | Art Sanella |
| 1987 | Jake and the Fatman | Mumbling Prisoner |
| 1987 | Penitentiary III | See Veer |
| 1987 | Terminal Exposure | man in crowd (uncredited) |
| 1985 | Põgenike rong | Boxer |
| 1975 | Saturday Night Live | Self - Cameo (uncredited) |
| 1972 | The Price Is Right | Self |
