| — | Empress of Serenity | |
| 2026 | The Cat in the Hat | The Cat in the Hat (voice) |
| 2024 | IF | Banana (voice) |
| 2024 | John Mulaney Presents: Everybody's in L.A. | Self |
| 2024 | Hendrie | Self |
| 2023 | Beau Is Afraid | UPS Guy |
| 2023 | Carol Burnett: 90 Years of Laughter + Love | Self - Guest |
| 2022 | Lightyear | Featheringhamstan (voice) |
| 2022 | Toast of Tinseltown | Dwight Difference |
| 2021 | The Addams Family 2 | Cyrus Strange (voice) |
| 2020 | Adventure Time: Distant Lands | Bufo (voice) |
| 2020 | Beastie Boys Story | "Crazy Shit" Voice |
| 2020 | Belushi | John Belushi (voice) |
| 2020 | Celebrity Escape Room | Self |
| 2019 | Toy Story 4 | Axel the Carnie (voice) |
| 2019 | It Chapter Two | Richie Tozier |
| 2019 | The Angry Birds Movie 2 | Leonard (voice) |
| 2019 | Noelle | Nick Kringle |
| 2019 | The Dark Crystal: Age of Resistance | urGoh (The Wanderer) (voice) |
| 2019 | The Summers of IT: Chapter Two | Self |
| 2019 | The Summers of IT: Chapter One | Self |
| 2019 | Desus & Mero | Self - Illustrious Guest |
| 2019 | Lights Out with David Spade | Self - Panelist |
| 2019 | At the Video Store | Self |
| 2018 | Ralph Breaks the Internet | J.P. Spamley (voice) (uncredited) |
| 2018 | Barry | Barry Berkman |
| 2018 | The Great Buster: A Celebration | Self |
| 2018 | Love, Gilda | Self |
| 2018 | Eli Roth's History of Horror | |
| 2018 | Eli Roth's History of Horror | Self |
| 2018 | The Shop | Self |
| 2018 | GQ Presents: Iconic Characters | Self |
| 2018 | Inside The Episode: Barry | Self |
| 2017 | Power Rangers | Alpha 5 (voice) |
| 2017 | Too Funny to Fail: The Life & Death of The Dana Carvey Show | Self |
| 2017 | The Power of the Present | Self - Voice of Alpha 5 (2017) |
| 2017 | Talking with Chris Hardwick | Self |
| 2017 | David Letterman: The Kennedy Center Mark Twain Prize | Self |
| 2016 | Finding Dory | Husband Fish (Stan) (voice) |
| 2016 | Sausage Party | Firewater / Tequila / El Guaco (voice) |
| 2016 | The Angry Birds Movie | Leonard (voice) |
| 2016 | The BFG | Bloodbottler |
| 2016 | Popstar: Never Stop Never Stopping | Zippy |
| 2016 | Maggie's Plan | Tony |
| 2016 | To Tell the Truth | Self - Panelist |
| 2016 | Neil Gaiman: Dream Dangerously | Self |
| 2016 | Bill Murray: The Kennedy Center Mark Twain Prize | Self |
| 2015 | Inside Out | Fear (voice) |
| 2015 | Trainwreck | Aaron Conners |
| 2015 | Star vs. the Forces of Evil | Additional Voices (voice) |
| 2015 | Riley's First Date? | Fear / Jordan's Joy (voice) |
| 2015 | Accidental Love | Doctor Turnstall |
| 2015 | Man Seeking Woman | Adolf Hitler |
| 2015 | Documentary Now! | Various Characters |
| 2015 | Close Up with The Hollywood Reporter | Self |
| 2015 | Beaver Trilogy Part IV | Narrator |
| 2015 | Mind Candy | Fear (voice) |
| 2015 | Saturday Night Live: 40th Anniversary Special | Self / Devin / Stefon |
| 2014 | 22 Jump Street | Culinary School Villain (uncredited) |
| 2014 | Silicon Valley | Pipey (voice) |
| 2014 | The Skeleton Twins | Milo Dean |
| 2014 | They Came Together | Kyle |
| 2014 | The Disappearance of Eleanor Rigby: Them | Stuart |
| 2014 | Clear History | Rags |
| 2014 | The Disappearance of Eleanor Rigby: Him | Stuart |
| 2014 | The Disappearance of Eleanor Rigby: Her | Stuart |
| 2014 | Late Night with Seth Meyers | Self |
| 2014 | Steve's First Bath | Flint Lockwood (voice) |
| 2014 | Variety Studio: Actors on Actors | Self |
| 2014 | Attack of the 50-foot Gummi Bear | Flint Lockwood (voice) |
| 2014 | Off Camera with Sam Jones | Self |
| 2013 | Her | Chat Room Friend #2 (voice) |
| 2013 | Monsters University | Referee / Slug (voice) |
| 2013 | Star Trek Into Darkness | Additional Voices (voice) |
| 2013 | Brooklyn Nine-Nine | Captain Seth Dozerman |
| 2013 | Turbo | Guy Gagné (voice) |
| 2013 | Cloudy with a Chance of Meatballs 2 | Flint Lockwood (voice) |
| 2013 | The To Do List | Willy Mclean |
| 2013 | Escape from Planet Earth | Announcer (voice) (uncredited) |
| 2013 | Comedy Central Roast of James Franco | Self |
| 2013 | Drunk History | John Pemberton |
| 2013 | The Awesomes | Dr. Malocchio (voice) |
| 2013 | Norm Macdonald Live | Self |
| 2013 | Here Comes the Night Time | Captain Bill the Astronaut |
| 2012 | Men in Black 3 | Andy Warhol |
| 2012 | This Is 40 | Desi's Store Pickup (uncredited) |
| 2012 | The Mindy Project | Tom McDougall |
| 2012 | Good Mythical Morning | Self |
| 2012 | Comedy Bang! Bang! | Self |
| 2012 | Finding Your Roots | Self |
| 2012 | Nantucket Film Festival's Comedy Roundtable | Self - Host |
| 2012 | The Secret Policeman's Ball | Self |
| 2011 | Paul | Haggard |
| 2011 | Bob's Burgers | Mickey (voice) |
| 2011 | Bob's Burgers | Big Bob Belcher (voice) |
| 2011 | Hoodwinked Too! Hood VS. Evil | Hansel (voice) |
| 2011 | Portlandia | Birdman |
| 2011 | 6 Days to Air: The Making of South Park | Self |
| 2011 | Billy on the Street | Self |
| 2011 | Saturday Night Live Backstage | Self / Stefon |
| 2010 | Scott Pilgrim vs. the World | The Voice (voice) |
| 2010 | Ugly Americans | William Dyer (voice) |
| 2010 | Conan | Self |
| 2010 | Saturday Night | Self |
| 2010 | Freaknik: The Musical | Tad (voice) |
| 2010 | The Women of SNL | Various (archive footage) |
| 2009 | Ice Age: Dawn of the Dinosaurs | Gazelle (voice) |
| 2009 | Night at the Museum: Battle of the Smithsonian | George Armstrong Custer |
| 2009 | Cloudy with a Chance of Meatballs | Flint Lockwood (voice) |
| 2009 | Adventureland | Bobby |
| 2009 | Year One | Shaman |
| 2009 | Late Night with Jimmy Fallon | Self |
| 2009 | Watch What Happens Live with Andy Cohen | Self - Guest |
| 2009 | SNL Presents: A Very Gilly Christmas | |
| 2009 | Saturday Night Live: The Best of Amy Poehler | Various (archive footage) |
| 2008 | Tropic Thunder | Rob Slolom |
| 2008 | Pineapple Express | Private Miller |
| 2008 | Forgetting Sarah Marshall | Brian Bretter |
| 2008 | Tropic Thunder: Rain of Madness | Rob Slolom |
| 2008 | Saturday Night Live Weekend Update Thursday | Various |
| 2007 | Superbad | Officer Slater |
| 2007 | Knocked Up | Brent |
| 2007 | Hot Rod | Dave |
| 2007 | The Brothers Solomon | Recumbent Biker |
| 2007 | Tim and Eric Awesome Show, Great Job! | Self as James Quall |
| 2007 | Purple Violets | Bill - Bookstore Fan |
| 2007 | Xavier: Renegade Angel | (voice) |
| 2007 | Human Giant | Little Kevin |
| 2007 | The Shooting | Keith |
| 2006 | You, Me and Dupree | Mark |
| 2006 | 30 Rock | Kevin |
| 2006 | Doogal | Soldier Sam (voice) |
| 2006 | The Pity Card | Eric |
| 2006 | Saturday Night Live: The Best of Saturday TV Funhouse | Self |
| 2005 | The Office | Bill Hader |
| 2005 | The Late Late Show with Craig Ferguson | Self |
| 2004 | The Venture Bros. | Professor Impossible / Phage (voice) |
| 2004 | The Venture Bros. | Professor Impossible (voice) |
| 2004 | The Venture Bros. | Professor Impossible / Alien villain (voice) |
| 2004 | Sounds Good to Me: Remastering 'The Sting' | Barnaby G. Price |
| 2003 | Punk'd | Field Agent |
| 2003 | Comedy Central Roasts | Self |
| 2002 | Collateral Damage | Pilot (deleted scene) |
| 2000 | Curb Your Enthusiasm | Igor, Gregor, & Timor |
| 1999 | Mark Twain Prize | Self |
| 1997 | South Park | Plex the robot |
| 1997 | South Park | Alec Baldwin |
| 1996 | The Daily Show | Self |
| 1996 | The Ambiguously Gay Duo | Self |
| 1996 | The Ambiguously Gay Duo | Flame Eye/Flatside (voice) |
| 1993 | Late Night with Conan O'Brien | Self - Guest |
| 1989 | The Simpsons | Manacek (voice) |
| 1989 | The Simpsons | Slava (voice) |
| 1975 | Saturday Night Live | Self - Various Characters |
| 1975 | Saturday Night Live | Self - Cameo (uncredited) |
| 1975 | Saturday Night Live | Self - Host |
| 1975 | Saturday Night Live | Robber |
| 1975 | Saturday Night Live | Talent Scout |
| 1975 | Saturday Night Live | |
| 1975 | Saturday Night Live | Illiterate Genie |
| 1975 | Saturday Night Live | Self / Nitro |
| 1975 | Saturday Night Live | Nitro |
| 1975 | Saturday Night Live | Dwight |
| 1975 | Saturday Night Live | Kevin |
| 1975 | Saturday Night Live | Self |
| 1975 | Saturday Night Live | Wizard |
| 1975 | Saturday Night Live | Life Coach |
| 1975 | Saturday Night Live | Frankenstein's Monster |
| 1975 | Saturday Night Live | Indiana Jones |
| 1975 | Saturday Night Live | Athlete |
| 1975 | Saturday Night Live | Gary |
| 1975 | Saturday Night Live | Self / Stefon |
| 1975 | Saturday Night Live | Cop |
